Typical Testing Data/Standard Curve (for reference only)
Human Trypsin ELISA Kit | PRSS1 elisa kit
Human Trypsin ELISA Kit
Gene Names
PRSS1; TRP1; TRY1; TRY4; TRYP1
Reactivity
Human
Synonyms
Trypsin; Human Trypsin ELISA Kit; TRP1; TRY1; TRYP1; Trypsin-1; Beta-trypsin; Cationic trypsinogen; Serine protease 1; Trypsin I; TRY2; TRYP2; Trypsin-2; Anionic trypsinogen; Serine protease 2; Trypsin II; PRSS4; TRY3; TRY4; Trypsin-3; Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV; PRSS1 elisa kit
Reactivity
Human
Specificity
The Human Trypsin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Trypsin proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
247
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<10 pg/mL
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRSS1 elisa kit
Principle of the Assay: MBS Human Trypsin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Trypsin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Trypsin coated on a 96-well plate. Standards and samples are pipetted into the wells and Trypsin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Trypsin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Trypsin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.
Background: Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,558 Da
NCBI Official Full Name
trypsin-1 preproprotein
NCBI Official Synonym Full Names
protease, serine 1
NCBI Official Symbol
PRSS1
NCBI Official Synonym Symbols
TRP1; TRY1; TRY4; TRYP1
NCBI Protein Information
trypsin-1
UniProt Protein Name
Trypsin-1
Protein Family
UniProt Gene Name
PRSS1
UniProt Synonym Gene Names
TRP1; TRY1; TRYP1
UniProt Entry Name
TRY1_HUMAN
NCBI Description
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]
Uniprot Description
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
Research Articles on PRSS1
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Product Notes
The Human PRSS1 prss1 (Catalog #AAA8248387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248387 ELISA Kit recognizes Human PRSS1. It is sometimes possible for the material contained within the vial of "Trypsin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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