Rabbit anti-Human USP18 Monoclonal Antibody | anti-USP18 antibody

USP18 (Ubiquitin Specific Protease 18, Ubiquitin-specific Protease 18, 43kD ISG15-specific Protease, ISG43, hUBP43, ISG15-specific-processing Protease, Ubl Carboxyl-terminal Hydrolase 18, Ubl Thioesterase 18)

Cat. Num. AAA605660

• Gene Names: USP18; ISG43; UBP43
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: USP18; Monoclonal Antibody; USP18 (Ubiquitin Specific Protease 18; Ubiquitin-specific Protease 18; 43kD ISG15-specific Protease; ISG43; hUBP43; ISG15-specific-processing Protease; Ubl Carboxyl-terminal Hydrolase 18; Ubl Thioesterase 18); Anti -USP18 (Ubiquitin Specific Protease 18; anti-USP18 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10g92
• Specificity: Recognizes endogenous levels of total USP18 protein. The doublet band detected by western blot represents full length (39kD) and amino-terminal deleted derivative of USP18 (8).
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-USP18 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Pro45 of human USP18 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from THP-1 cells, untreated or LPS-treated (1ug/ml, 24 hours), using MBS605660)

Related Product Information for anti-USP18 antibody

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes (1,2). USP18 (also known as UBP43) is a deubiquitinase best known for catalyzing the removal of ISG15, an interferon-regulated ubibiquitin-like protein, from conjugated proteins (3). Removal of ISG15 from target proteins by the USP18 peptidase maintains the critical cellular balance of ISG15-conjugated proteins important for normal development and brain function (4,5). Following induction by IFN or LPS (6), USP18 binds the INF receptor subunit IFNAR2 and inhibits signal transduction through the JAK-STAT pathway (7). USP18 regulation of IFN signaling inhibits IFN-mediated apoptosis and does not necessarily rely on USP18 peptidase activity (8). As the therapeutic use of recombinant IFN can lead to refractory IFN signaling and a less effective response, the combination of IFN treatment and regulation of USP18 expression may produce a more positive outcome (9).

Product Categories/Family for anti-USP18 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 32313610
• NCBI GeneID: 11274
• NCBI Accession #: NP_059110.2
• NCBI GenBank Nucleotide #: NM_017414.3
• UniProt Accession #: Q53Y90; Q9UMW8
• Molecular Weight: 43,011 Da
• NCBI Official Full Name: ubl carboxyl-terminal hydrolase 18
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 18
• NCBI Official Symbol: USP18
• NCBI Official Synonym Symbols: ISG43; UBP43
• NCBI Protein Information: ubl carboxyl-terminal hydrolase 18; hUBP43; OTTHUMP00000196334; ubl thioesterase 18; ubl thiolesterase 18; 43 kDa ISG15-specific protease; ubiquitin specific protease 18; ISG15-specific-processing protease
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase
• Protein Family: Ubl carboxyl-terminal hydrolase
• UniProt Gene Name: Em:AC008079.1
• UniProt Entry Name: Q53Y90_HUMAN

NCBI Description

USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM]

Uniprot Description

USP18: Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Belongs to the peptidase C19 family.

Protein type: Protease; EC 3.4.19.-; Ubiquitin-specific protease; EC 3.1.2.-

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; ISG15-specific protease activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; cytokine and chemokine mediated signaling pathway