Western Blot (WB)
(The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PRPS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysate)
Mouse anti-Human PRPS1 Monoclonal Antibody | anti-PRPS1 antibody
PRPS1 antibody
Gene Names
PRPS1; ARTS; DFN2; PRSI; CMTX5; DFNX1; PRS-I; PPRibP
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
PRPS1; Monoclonal Antibody; PRPS1 antibody; Ribose-phosphate pyrophosphokinase 1; ARTS; CMTX5; PPRibP; PRSI; DFN2; DFNX1; Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1; KIAA0967; Phosphoribosyl pyrophosphate synthetase I; PRS I; PRS-I; Ribose phosphate pyrophosphokinase I; ; anti-PRPS1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
AT1E11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
318
Applicable Applications for anti-PRPS1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
Recombinant human PRPS1 (1-318aa) purified from E. coli
Preparation and Storage
Can be stored at 2-8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PRPS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysate)
Western Blot (WB)
(The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PRPS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of PRPS1 in HeLa cells. The cell was stained with MBS200257 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunofluorescence (IF)
(ICC/IF analysis of PRPS1 in HeLa cells. The cell was stained with MBS200257 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-PRPS1 antibody
PRPS1 is an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. A mutation in PRPS1 may result in PRPS superactivity, a disease characterized by gout and the overproduction of purine nucleotides, uric acid and PRPP. PRPS1 mutations can also lead to a reduction in PRPS1 activity resulting in ARTS syndrome or CMTX5 (Charcot-Marie-Tooth disease X-linked recessive type 5).
Product Categories/Family for anti-PRPS1 antibody
References
Ishizuka T, et al. (1992) Biochim Biophsy Acta 24;1130 (2): 139-48; Kim HJ, et al. (2007) Arn J Hum Gernet 81 (3):552-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ribose-phosphate pyrophosphokinase 1 isoform 1
NCBI Official Synonym Full Names
phosphoribosyl pyrophosphate synthetase 1
NCBI Official Symbol
PRPS1
NCBI Official Synonym Symbols
ARTS; DFN2; PRSI; CMTX5; DFNX1; PRS-I; PPRibP
NCBI Protein Information
ribose-phosphate pyrophosphokinase 1
UniProt Protein Name
Ribose-phosphate pyrophosphokinase 1
UniProt Gene Name
PRPS1
UniProt Synonym Gene Names
PRS-I
NCBI Description
This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
Research Articles on PRPS1
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Product Notes
The PRPS1 prps1 (Catalog #AAA200257) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRPS1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRPS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PRPS1 prps1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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