Chloromethylketone FPR, Protein
Chloromethylketone FPR, (FPRCK) (Biotin)
Applications
ELISA, Western Blot, Immunofluorescence
Purity
PurifiedHPLC: 80%
Synonyms
Chloromethylketone FPR; ; (FPRCK) (Biotin); protein
Purity/Purification
Purified
HPLC: 80%
HPLC: 80%
Form/Format
Supplied as a liquid in 10mM HCl. Labeled with Biotin.
Applicable Applications for Chloromethylketone FPR, protein
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Biotinylated tripeptide chloromethyl ketones can be used in a variety of ways (11-13). First, the compounds can be reacted with unwanted serine proteases in a sample or preparation, and can then be removed along with the protease using avidin-Sepharose (11). Second, the biotinylated-serine protease can be visualized on a blot without the use of specific antibodies (11). Third, the biotinylated serine protease can be quantitated in an active-site specific immunoassay (12,13). The spacer utilized on these compounds has been optimized to allow good reactivity of the biotinylated FPRCK in the above mentioned procedures., In addition to biotinylated chloromethyl ketones, fluorescein labeled compounds are also available. The fluorescein labeled compounds are useful in both Western blot and fluorescent imaging applications.
Molecular Formula
C46H72O7N10SCl3
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Related Product Information for Chloromethylketone FPR, protein
FPRCK labeled with biotin is prepared by the method of Williams, et al. (11).
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common chloromethylketones are FPRCK (Phe-Pro-Arg-chloromethylketone), which is a rapid inhibitor of a-thrombin and EGRCK (Glu-Gly-Arg-chloromethylketone), which rapidly inhibits factor Xa (1). Recently, the modification of these tri-peptide chloromethylketones with reporting groups, such as fluorescent probes (6-8), radioactive labels (9) or thioreactive-labels (10), has provided a unique approach to the study of various serine proteases. These probes are useful because they allow a means of reporting molecular changes in an enzyme, and not its zymogen, while also inhibiting the enzymatic activity.
The use of biotin as a reporting group has been used extensively with antibodies in ELISA based assays and in Western Blot. The biotin, in conjunction with avidin, creates a highly sensitive method for detecting antibodies, and therefore, antigens. By modifying the tripeptide-chloromethylketones with a biotin group, the sensitivity of the avidin/biotin system can be extended to study serine proteases without the need for specific antibodies to the active enzymes.
Tri-peptide chloromethylketones have been utilized extensively to irreversibly inhibit various serine proteases (1-5). Among the most common chloromethylketones are FPRCK (Phe-Pro-Arg-chloromethylketone), which is a rapid inhibitor of a-thrombin and EGRCK (Glu-Gly-Arg-chloromethylketone), which rapidly inhibits factor Xa (1). Recently, the modification of these tri-peptide chloromethylketones with reporting groups, such as fluorescent probes (6-8), radioactive labels (9) or thioreactive-labels (10), has provided a unique approach to the study of various serine proteases. These probes are useful because they allow a means of reporting molecular changes in an enzyme, and not its zymogen, while also inhibiting the enzymatic activity.
The use of biotin as a reporting group has been used extensively with antibodies in ELISA based assays and in Western Blot. The biotin, in conjunction with avidin, creates a highly sensitive method for detecting antibodies, and therefore, antigens. By modifying the tripeptide-chloromethylketones with a biotin group, the sensitivity of the avidin/biotin system can be extended to study serine proteases without the need for specific antibodies to the active enzymes.
Product Categories/Family for Chloromethylketone FPR, protein
Similar Products
Product Notes
The Chloromethylketone FPR, (Catalog #AAA635026) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Chloromethylketone FPR, can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Biotinylated tripeptide chloromethyl ketones can be used in a variety of ways (11-13). First, the compounds can be reacted with unwanted serine proteases in a sample or preparation, and can then be removed along with the protease using avidin-Sepharose (11). Second, the biotinylated-serine protease can be visualized on a blot without the use of specific antibodies (11). Third, the biotinylated serine protease can be quantitated in an active-site specific immunoassay (12,13). The spacer utilized on these compounds has been optimized to allow good reactivity of the biotinylated FPRCK in the above mentioned procedures., In addition to biotinylated chloromethyl ketones, fluorescein labeled compounds are also available. The fluorescein labeled compounds are useful in both Western blot and fluorescent imaging applications. Researchers should empirically determine the suitability of the Chloromethylketone FPR, for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chloromethylketone FPR,, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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