Rabbit anti-Human, Mouse KLRK1 Polyclonal Antibody | anti-KLRK1 antibody

Anti-KLRK1 antibody

Cat. Num. AAA7611948

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Synonyms: KLRK1; Polyclonal Antibody; Anti-KLRK1 antibody; CD314; D12S2489E; KLR; NKG2-D; NKG2D; anti-KLRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Form/Format: Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3

• Applicable Applications for anti-KLRK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: NKG2-D type II integral membrane protein
• Preparation and Storage: Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7611948(KLRK1 Antibody) at dilution of 1:1000)

Related Product Information for anti-KLRK1 antibody

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

NCBI and Uniprot Product Information

• NCBI GI #: 315221164
• NCBI GeneID: 100528032
• NCBI Accession #: NP_001186734.1
• NCBI GenBank Nucleotide #: NM_001199805.1
• UniProt Accession #: P26718; Q9NR41; A8K7K5; A8K7P4
• Molecular Weight: 25,274 Da
• NCBI Official Full Name: NKG2-D type II integral membrane protein
• NCBI Official Symbol: KLRC4-KLRK1
• NCBI Protein Information: NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor; killer cell lectin-like receptor subfamily K member 1
• UniProt Protein Name: NKG2-D type II integral membrane protein
• Protein Family: NKG2-D type II integral membrane protein
• UniProt Gene Name: KLRK1
• UniProt Synonym Gene Names: D12S2489E; NKG2D
• UniProt Entry Name: NKG2D_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]

Uniprot Description

KLRK1: Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: regulation of immune response; T cell costimulation; natural killer cell activation; innate immune response; cell differentiation; positive regulation of natural killer cell mediated cytotoxicity; signal transduction