Prominin 1 (PROM1) Recombinant Protein | PROM1 recombinant protein

Recombinant Prominin 1 (PROM1)

Cat. Num. AAA2009551

• Gene Names: Prom1; Prom; AC133; CD133; Prom-1; Proml1; 4932416E19Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Prominin 1 (PROM1); Recombinant Prominin 1 (PROM1); PROM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-A NQQTRTRIKG TQKLAKSNFR DFQTLLTETP KQIDYVVEQY TNTKNKAFSD LDGIGSVLGG RIKDQLKPKV TPVLEEIKAM ATAIKQTKDA LQNMSSSLKS LQDAATQLNT NLSSVRNSIE NSLSSSDCTS DPASKICDSI RPSLSSLGSS LNSSQLPSVD RELNTVTEVD KTDLESLVKR GYTTIDEIPN TIQNQTVDVI KDVKNTLDSI SSNIKDMSQS IPIEDMLLQV SHYLNNSNRY LNQELPKLEE YDSYW
• Sequence Length: 834

• Applicable Applications for PROM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala180~Trp435 (Accession # O54990) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 254675304
• NCBI GeneID: 19126
• NCBI Accession #: NP_001157050.1
• NCBI GenBank Nucleotide #: NM_001163578.1
• UniProt Accession #: O54990; O35408; Q80XB2; Q80XB3; Q80XB6; Q8BH12
• Molecular Weight: 29.7kDa
• NCBI Official Full Name: prominin-1 isoform s3
• NCBI Official Synonym Full Names: prominin 1
• NCBI Official Symbol: Prom1
• NCBI Official Synonym Symbols: Prom; AC133; CD133; Prom-1; Proml1; 4932416E19Rik
• NCBI Protein Information: prominin-1; prominin-like 1; antigen AC133 homolog; prominin-like protein 1
• UniProt Protein Name: Prominin-1
• Protein Family: Prominin
• UniProt Gene Name: Prom1
• UniProt Synonym Gene Names: Prom; Proml1
• UniProt Entry Name: PROM1_MOUSE

Uniprot Description

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: extracellular space; microvillus; cell surface; endoplasmic reticulum; integral to plasma membrane; integral to membrane; ER-Golgi intermediate compartment; cilium; stereocilium; photoreceptor outer segment; cell projection; membrane; apical plasma membrane; plasma membrane; brush border; vesicle

Molecular Function: actinin binding; cadherin binding

Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye