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Western Blot (WB) (Anti-PROM1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse Kidney Tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:97 kDaObserved band size:120 kDa)

Rabbit anti-Mouse CD133/PROM1 Polyclonal Antibody | anti-PROM1 antibody

Anti-CD133/PROM1 Antibody, Rabbit Polyclonal

Gene Names
Prom1; Prom; AC133; CD133; Prom-1; Proml1; 4932416E19Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD133/PROM1; Polyclonal Antibody; Anti-CD133/PROM1 Antibody; Rabbit Polyclonal; PROM1 Antibody; Rabbit PAb; Antigen Affinity Purified; prominin 1; Anti-4932416E19Rik Antibody; Anti-AC133 Antibody; Anti-CD133 Antibody; Anti-Prom Antibody; Anti-Prom-1 Antibody; Anti-Proml1 Antibody; anti-PROM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse PROM1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PROM1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Mouse PROM1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse PROM1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PROM1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse Kidney Tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:97 kDaObserved band size:120 kDa)

Western Blot (WB) (Anti-PROM1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse Kidney Tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:97 kDaObserved band size:120 kDa)
Related Product Information for anti-PROM1 antibody
CD133, also known as PROM1 and Prominin 1, is a pentaspan transmembrane glycoprotein that belongs to the prominin family. It localizes to membrane protrusions and is often expressed on adult stem cells. CD133 is known to play a role in maintaining stem cell properties by suppressing differentiation. CD133 binds cholesterol in cholesterol-containing plasma membrane microdomains. It is proposed to play a role in apical plasma membrane organization of epithelial cells. CD133 is also involved in regulation of MAPK and Akt signaling pathways. Mutations in PROM1 gene have been shown to result in retinitis pigmentosa and Stargardt disease. PROM1 gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Expression of this gene is also associated with several types of cancer.
Product Categories/Family for anti-PROM1 antibody
References
Corbeil D. et al., 2001, Biochem Biophys Res Commun. 285 (4): 939-44. Horn PA. et al., 1999, Blood. 93 (4): 1435-37. Sanai N. et al., 2005, N Engl J Med. 353 (8): 811-22.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
92,197 Da
NCBI Official Full Name
Prominin-1
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
Prom1
NCBI Official Synonym Symbols
Prom; AC133; CD133; Prom-1; Proml1; 4932416E19Rik
NCBI Protein Information
prominin-1; prominin-like 1; antigen AC133 homolog; prominin-like protein 1
UniProt Protein Name
Prominin-1
Protein Family
UniProt Gene Name
Prom1
UniProt Synonym Gene Names
Prom; Proml1
UniProt Entry Name
PROM1_MOUSE

Uniprot Description

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell surface; Membrane protein, integral

Cellular Component: extracellular space; microvillus; cell surface; integral to plasma membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane; cilium; stereocilium; photoreceptor outer segment; cell projection; membrane; apical plasma membrane; plasma membrane; brush border; vesicle

Molecular Function: actinin binding; cadherin binding

Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye

Research Articles on PROM1

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Product Notes

The PROM1 prom1 (Catalog #AAA8101600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD133/PROM1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD133/PROM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PROM1 prom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133/PROM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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