Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

CD4 Antibody [8G1B12]

Cat. Num. AAA150003

• Gene Names: CD4; CD4mut
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Synonyms: CD4; Monoclonal Antibody; CD4 Antibody [8G1B12]; CD4 Antibody [8G1B12] : CD4mut; CD4 molecule; anti-CD4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8G1B12
• Purity/Purification: CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CD4 antibody can be used for detection of CD4 by Western blot at 0.5 - 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
• Buffer: CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CD4 monoclonal antibody can be stored at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of CD4 in rat lung tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.Green: CD4 Antibody [8G1B12]Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-CD4 antibody

CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; enzyme binding; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; signal transduction; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation

Disease: Okt4 Epitope Deficiency