Protein C (PROC) Recombinant Protein | PROC recombinant protein

Recombinant Protein C (PROC)

Cat. Num. AAA2011412

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein C (PROC); Recombinant Protein C (PROC); PROC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- WKRTD KKRKNFKRDI DPEDEELELG PRIVNGTLTK QGDSPWQAIL LDSKKKLACG GVLIHTSWVL TAAHCLESSK KLTVRLGEYD LRRRDPWELD LDIKEVLVHP NYTRSNSDND IALLRLSQPA TLSKTIVPIC LPNSGLAQEL SQAGQETVVT GWGYQSDKVK DGRRNRTFIL TFIRIPLAAR NDCMQVMNNV VSENMLCAGI IGDTRDACDG DSGGPMVVFF RGTWFLVGLVSWGEGCGHLN NYGVYTK
• Sequence Length: 461

• Applicable Applications for PROC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Trp186~Lys437 (Accession # P31394) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.5
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6981412
• NCBI GeneID: 25268
• NCBI Accession #: NP_036935.1
• NCBI GenBank Nucleotide #: NM_012803.1
• UniProt Accession #: P31394
• Molecular Weight: 55.2kDa
• NCBI Official Full Name: vitamin K-dependent protein C
• NCBI Official Synonym Full Names: protein C
• NCBI Official Symbol: Proc
• NCBI Protein Information: vitamin K-dependent protein C; autoprothrombin IIA; anticoagulant protein C; blood coagulation factor XIV
• UniProt Protein Name: Vitamin K-dependent protein C
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: Proc
• UniProt Entry Name: PROC_RAT

NCBI Description

vitamin K-dependent zymogen that acts as a serine protease upon activation; catalyzes the inactivation of blood coagulation cofactors Va and VIIIa [RGD, Feb 2006]

Uniprot Description

PROC: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids

Protein type: Protease; Apoptosis; EC 3.4.21.69

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; serine-type endopeptidase activity; calcium ion binding

Biological Process: negative regulation of inflammatory response; negative regulation of blood coagulation; negative regulation of coagulation; blood coagulation; proteolysis; negative regulation of apoptosis