Mouse anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody

Anti-CD33 [M195]

Cat. Num. AAA488860

• Gene Names: CD33; p67; SIGLEC3; SIGLEC-3
• Reactivity: Human
• Applications: immunoassay, Radioimmunoassay, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD33; Monoclonal Antibody; Anti-CD33 [M195]; gp67; Siglec-3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; anti-CD33 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone Number: M195
• Specificity: This antibody binds CD33, which is a 67 kD glycoprotein expressed on monocytes, hematopoetic progenitors, early myeloid progenitor cells and myeloid leukemias (acute and chronic) but not expressed on mature myeloid cells, normal stem cells, other hemtopoietic cells and non hematopoietic human tissues.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD33 antibody: Radioimmunoassay (RIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Therapeutics
• Application Notes: This antibody is recommended for myeloid differentiation studies, test of acute non-lymphoid leukemia cells and therapeutic treatment of myelois leukemia. Specificity of this antibody to CD33 was determined using RIA and immunoabsorption. Immunoflurorescence assay showed that the antigen is not expressed on adult human tissues (PMID: 2716349). Flow cytometric analysis was used to confirm reactivity of M195 with fresh hematopoietic neoplasms in the blood or bone marrow. In combination with MY9, this antibody is useful in test of acute myelogenous leukemia(AML) (PMID: 2725060). Murine antibody M195 was used in the phase I trial of AML (PMID: 1999719). Humanized IgG1 and IgG3 versions of this antibody has higher apparent binding affinity for CD33 antigen than the chimeric and parent murine version (PMID: 1737932, 1458463). The increase in binding affinity resulted from eliminating an N-linked glycosylation site at residue 73 in the heavy chain variable region in the course of humanization (PMID: 8232322). Preliminary trials to treat AML with humanized antibody showed specific bone marrow targeting without an immunogenic response (PMID: 8124221).
• PMID: 2716349
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed U937 cells with anti-CD33 antibody M195 (MBS488861) Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of M195 (MBS488861) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488861, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CD33 antibody

Recombinant monoclonal antibody to CD33. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma M195.
The original antibody was generated by immunizing mice with live leukemic myeloblasts.

Product Categories/Family for anti-CD33 antibody

cancer; oncology; therapeutic

References

Tanimoto et al. Restricted Expression of an Early Myeloid and Monocytic Cell Surface Antigen Defined by Monoclonal Antibody M195. Leukemia (1989); 3(5):339-48.
Note on publication: Describes the restricted expression of a myeloid and monocytic cell surface antigen and its charaterization with this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 116241290
• NCBI GeneID: 945
• UniProt Accession #: P20138; Q8TD24; B4E3P8; C9JEN7; F8WAL2
• Molecular Weight: Calculated MW: 25kDa/33kDa/39kDa; Observed MW: 77kDa
• NCBI Official Full Name: Myeloid cell surface antigen CD33
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
• UniProt Protein Name: Myeloid cell surface antigen CD33
• Protein Family: Myeloid cell surface antigen
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3; Siglec-3
• UniProt Entry Name: CD33_HUMAN

Uniprot Description

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion