Chicken Major prion protein ELISA Kit | PRNP elisa kit

Chicken Major prion protein homolog ELISA Kit

Cat. Num. AAA2887621

• Gene Names: PRNP; prp; Prn-p
• Reactivity: Chicken
• Synonyms: Major prion protein; Chicken Major prion protein homolog ELISA Kit; Major prion protein homolog; 65-21 protein; Acetylcholine receptor-inducing activity; ARIA; PR-LP; PRNP; PRN-P; PRNP elisa kit

• Reactivity: Chicken
• Specificity: This assay recognizes recombinant and natural chicken Major prion protein homolog. No significant cross-reactivity or interference was observed.
• Sequence Length: 273

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.31 ng/mL - 20.0 ng/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRNP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of chicken Major prion protein homolog concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major prion protein homolog. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Major prion protein homolog and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Major prion protein homolog, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Major prion protein homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 424036619
• NCBI GeneID: 396452
• NCBI Accession #: NP_990796.2
• NCBI GenBank Nucleotide #: NM_205465.2
• UniProt Accession #: P27177
• Molecular Weight: 29,909 Da
• NCBI Official Full Name: major prion protein homolog preproprotein
• NCBI Official Synonym Full Names: prion protein
• NCBI Official Symbol: PRNP
• NCBI Official Synonym Symbols: prp; Prn-p
• NCBI Protein Information: major prion protein homolog
• UniProt Protein Name: Major prion protein homolog
• Protein Family: Major prion protein
• UniProt Gene Name: PRNP
• UniProt Synonym Gene Names: PRN-P; ARIA
• UniProt Entry Name: PRIO_CHICK

NCBI Description

This gene encodes a membrane glycosylphosphatidylinositol-anchored glycoprotein found in the central nervous system. In human and other vertebrates, the homologous protein is associated with neurodegenerative diseases that result from improper protein metabolism. [provided by RefSeq, Nov 2012]

Uniprot Description

Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().