SDS-Page
(Recombinant PRMT5 / MEP50 complex - protein gel. 10% SDS-PAGE gel stained with Coomassie blue. MW of PRMT5: 74 kDa MW of MEP50: 36.7 kDa Purity: > 90% )
PRMT5/MEP50 recombinant protein
Recombinant PRMT5/MEP50 complex
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PRMT5 / MEP50 complex - protein gel. 10% SDS-PAGE gel stained with Coomassie blue. MW of PRMT5: 74 kDa MW of MEP50: 36.7 kDa Purity: > 90% )
SDS-Page
(Recombinant PRMT5 / MEP50 complex - protein gel. 10% SDS-PAGE gel stained with Coomassie blue. MW of PRMT5: 74 kDa MW of MEP50: 36.7 kDa Purity: > 90% )
Western Blot (WB)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
Western Blot (WB)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. PRMT5 acts as part of a multimeric complex in concert with a variety of partner proteins that regulate its function and specificity. A core component of these complexes is the WD40 protein MEP50/WDR77/p44, which mediates interactions with binding partners and substrates. PRMT5 is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Uniprot Description
PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity
Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation
Research Articles on PRMT5/MEP50
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Product Notes
The PRMT5/MEP50 prmt5 (Catalog #AAA388225) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRMT5/MEP50, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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