5'-AMP-activated protein kinase subunit gamma-2 (PRKAG2) Recombinant Protein | PRKAG2 recombinant protein

Recombinant Human 5'-AMP-activated protein kinase subunit gamma-2 (PRKAG2)

Cat. Num. AAA1409676

• Gene Names: PRKAG2; AAKG; CMH6; WPWS; AAKG2; H91620p
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 5'-AMP-activated protein kinase subunit gamma-2 (PRKAG2); Recombinant Human 5'-AMP-activated protein kinase subunit gamma-2 (PRKAG2); 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; H91620p; PRKAG2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-328aa; Full Length of Isoform B
• Sequence: MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
• Sequence Length: 569

• Species: Homo sapiens (Human)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Related Product Information for PRKAG2 recombinant protein

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Product Categories/Family for PRKAG2 recombinant protein

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 100913192
• NCBI GeneID: 51422
• NCBI Accession #: NP_001035723.1
• NCBI GenBank Nucleotide #: NM_001040633.1
• UniProt Accession #: Q9UGJ0; Q53Y07; Q6NUI0; Q75MP4; Q9NUZ9; Q9UDN8; Q9ULX8
• Molecular Weight: 41.5 kDa
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-2 isoform c
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• NCBI Official Symbol: PRKAG2
• NCBI Official Synonym Symbols: AAKG; CMH6; WPWS; AAKG2; H91620p
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-2; AMPK subunit gamma-2; hypothetical protein
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAG2
• UniProt Synonym Gene Names: AMPK gamma2; AMPK subunit gamma-2
• UniProt Entry Name: AAKG2_HUMAN

NCBI Description

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; extracellular space; cytosol; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; ADP binding; phosphorylase kinase regulator activity; protein kinase binding; protein kinase activator activity; ATP binding; AMP binding

Biological Process: glycogen metabolic process; mitochondrion organization and biogenesis; organelle organization and biogenesis; carnitine shuttle; cellular lipid metabolic process; protein amino acid phosphorylation; regulation of fatty acid biosynthetic process; positive regulation of protein kinase activity; ATP biosynthetic process; regulation of glycolysis; sterol biosynthetic process; regulation of fatty acid metabolic process; insulin receptor signaling pathway; energy reserve metabolic process; regulation of fatty acid oxidation; negative regulation of protein kinase activity; regulation of glucose import; cell cycle arrest; fatty acid biosynthetic process

Disease: Glycogen Storage Disease Of Heart, Lethal Congenital; Cardiomyopathy, Familial Hypertrophic, 6; Wolff-parkinson-white Syndrome