Guinea Pig chemokine (C-C motif) ligand 2 ELISA Kit | CCL2 elisa kit

Guinea Pig monocyte chemotactic protein 1, MCP1 ELISA Kit

Cat. Num. AAA701253

• Gene Names: Ccl2; MCP1; MCP-1; SCYA2
• Reactivity: Guinea Pig
• Synonyms: chemokine (C-C motif) ligand 2; Guinea Pig monocyte chemotactic protein 1; MCP1 ELISA Kit; Guinea pig monocyte chemotactic protein 1; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; SCYA2; SMC-CF; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine; CCL2 elisa kit

• Reactivity: Guinea Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Guinea pig CCL2. No significant cross-reactivity or interference between Guinea pig CCL2 and analogues was observed.
• Sequence Length: 120

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: 5.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 2: chemokine
• Protein Biological Process 3: Chemotaxis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL2 elisa kit

Introduction: Chemokine (C-C motif) ligand 2 (MCP-1) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). MCP-1 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, MCP-1 is located on chromosome 17 in humans. The cell surface receptors that bind MCP-1 are CCR2 and CCR4. It is found at the site of tooth eruption and bone degradation. In the bone, MCP-1 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappaB (NFkappaB). In human osteoclasts, it has been shown that MCP-1 and RANTES (regulated on activation normal T cell expressed and secreted) are unregulated by RANKL (receptor activator of NFkappaB ligand). Both MCP-1 and RANTES were also shown to induce the formation of TRAP-positive, multinuclear cells from M-CSF-treated monocytes in the absence of RANKL, but produced osteoclasts that lacked cathepsin K expression and resorptive capacity. It is proposed that MCP-1 and RANTES act as autocrine loop in human osteoclast differentiation. MCP-1 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MCP-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MCP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 290543400
• NCBI GeneID: 100135494
• NCBI Accession #: NP_001166397.1
• NCBI GenBank Nucleotide #: NM_001172926.1
• UniProt Accession #: Q08782
• Molecular Weight: 13,741 Da
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: MCP1; MCP-1; SCYA2
• NCBI Protein Information: C-C motif chemokine 2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: MCP1; SCYA2; MCP-1
• UniProt Entry Name: CCL2_CAVPO

Uniprot Description

Function: Chemotactic factor that attracts monocytes, but not neutrophils.