Peroxiredoxin 5 (PRDX5) Recombinant Protein | PRDX5 recombinant protein

Recombinant Peroxiredoxin 5 (PRDX5)

Cat. Num. AAA2011100

• Gene Names: Prdx5; Aoeb166
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxiredoxin 5 (PRDX5); Recombinant Peroxiredoxin 5 (PRDX5); PRDX5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MVQLRFCVLG SIAGSVLRAS ATWTCVAGRA GRKGAGWECG GARSFSSAAV TMAPIKVGDT IPSVEVFEGE PGKKVNLAEL FKDKKGVLFG VPGAFTPGCS KTHLPGFVEQ AGALKAKGAQ VVACLSVNDA FVTAEWGRAH QAEGKVQLLA DPTGAFGKET DLLLDDSLVS LFGNRRLKRF SMVIDKGVVK ALNVEPDGTG LTCSLAPNIL SQL
• Sequence Length: 213

• Applicable Applications for PRDX5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu213 (Accession # Q9R063) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 16758404
• NCBI GeneID: 113898
• NCBI Accession #: NP_446062.1
• NCBI GenBank Nucleotide #: NM_053610.1
• UniProt Accession #: Q9R063; Q68G22
• Molecular Weight: 23.7kDa
• NCBI Official Full Name: peroxiredoxin-5, mitochondrial
• NCBI Official Synonym Full Names: peroxiredoxin 5
• NCBI Official Symbol: Prdx5
• NCBI Official Synonym Symbols: Aoeb166
• NCBI Protein Information: peroxiredoxin-5, mitochondrial; PLP; prx-V; peroxiredoxin V; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; peroxisomal antioxidant enzyme
• UniProt Protein Name: Peroxiredoxin-5, mitochondrial
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx5
• UniProt Synonym Gene Names: AOEB166; Prx-V
• UniProt Entry Name: PRDX5_RAT

NCBI Description

member of the AhpC/TSA peroxiredoxin family with mitochondrial and peroxisomal sorting signals; may play an antioxidant protective role in various tissues [RGD, Feb 2006]

Uniprot Description

PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Oxidoreductase; EC 1.11.1.15

Cellular Component: peroxisomal matrix; extracellular space; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; oxidoreductase activity; receptor binding

Biological Process: hydrogen peroxide catabolic process; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase III promoter; negative regulation of oxidoreductase activity; response to oxidative stress; negative regulation of caspase activity; negative regulation of apoptosis