Mouse PR domain containing 16 ELISA Kit | PRDM16 elisa kit

Mouse PR domain zinc finger protein 16, PRDM16 ELISA Kit

Cat. Num. AAA906925

• Gene Names: Prdm16; csp1; mel1; 5730557K01Rik
• Reactivity: Mouse
• Synonyms: PR domain containing 16; Mouse PR domain zinc finger protein 16; PRDM16 ELISA Kit; Mouse PR domain zinc finger protein 16 (PRDM16) ELISA kit; KIAA1675; MEL1; MGC166915; PFM13; MDS1/EVI1-like; PR-domain zinc finger protein 16; transcription factor MEL1; PRDM16 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PRDM16. No significant cross-reactivity or interference between mouse PRDM16 and analogues was observed.
• Sequence Length: 1274

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse PRDM16 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRDM16 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRDM16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDM16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 597517895
• NCBI GeneID: 70673
• NCBI Accession #: NP_001277955.1
• NCBI GenBank Nucleotide #: NM_001291026.1
• UniProt Accession #: A2A935; Q69ZD6; Q6PB79; Q7TPF4
• Molecular Weight: 140,858 Da
• NCBI Official Full Name: PR domain zinc finger protein 16 isoform 3
• NCBI Official Synonym Full Names: PR domain containing 16
• NCBI Official Symbol: Prdm16
• NCBI Official Synonym Symbols: csp1; mel1; 5730557K01Rik
• NCBI Protein Information: PR domain zinc finger protein 16; line 27; MDS1/EVI1-like gene 1; transcription factor MEL1; PR domain-containing protein 16
• UniProt Protein Name: PR domain zinc finger protein 16
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: Prdm16
• UniProt Synonym Gene Names: Kiaa1675; Mel1; MDS1/EVI1-like gene 1
• UniProt Entry Name: PRD16_MOUSE

Uniprot Description

PRDM16: binds DNA and functions as a transcriptional regulator. A repressor of TGF-beta signaling. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. Four human isoforms are produced by alternative promoter usage and alternative splicing

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: protein binding; nucleic acid binding; transcription activator binding; DNA binding; sequence-specific DNA binding; metal ion binding; transcription coactivator activity; SMAD binding

Biological Process: tongue development; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of granulocyte differentiation; regulation of cellular respiration; neurogenesis; regulation of transcription, DNA-dependent; white fat cell differentiation; brown fat cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; cell differentiation