SDS-Page
(#ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
AKR1B10 active protein
AKR1B10, 1-316aa, Human, E Coli (Bioactivity Validated)
Gene Names
AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
AKR1B10; 1-316aa; Human; E Coli (Bioactivity Validated); Aldo-keto reductase family 1 member B10; AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; Aldo-keto reductase family 1; member B10; member B10 Aldose reductase like; Aldose reductase related protein; ARL 1; hARP; SI reductase; Small intestine reductase.; AKR1B10 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
Sequence Length
316
Applicable Applications for AKR1B10 active protein
SDS-PAGE, Enzyme Activity
Tag
Non-Tagged
Biological Activity
Specific activity is > 1,500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340nm resulting from NADPH at pH 7.0 at 37C.
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze and thawing cycles.
SDS-Page
(#ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(#ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for AKR1B10 active protein
AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for AKR1B10 active protein
References
Yabe D., et al. (1997) J Biol Chem. 272 (29):18232-9.; Vorum H., et al. (1998) Biochim Biophys Acta. 1386 (1):121-31.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36 kDa (316aa), confirmed by MALDI-TOF.
NCBI Official Full Name
aldo-keto reductase family 1 member B10
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member B10
NCBI Official Symbol
AKR1B10
NCBI Official Synonym Symbols
HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
NCBI Protein Information
aldo-keto reductase family 1 member B10
UniProt Protein Name
Aldo-keto reductase family 1 member B10
Protein Family
UniProt Gene Name
AKR1B10
UniProt Synonym Gene Names
AKR1B11; ARP; hARP; SI reductase
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Research Articles on AKR1B10
Similar Products
Product Notes
The AKR1B10 akr1b10 (Catalog #AAA203315) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AKR1B10 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Enzyme Activity. Researchers should empirically determine the suitability of the AKR1B10 akr1b10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY. It is sometimes possible for the material contained within the vial of "AKR1B10, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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