Human PR domain zinc finger protein 1 ELISA Kit | PRDM1 elisa kit
Human PR domain zinc finger protein 1 (PRDM1) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRDM1 in Human serum, plasma. An antibody specific for PRDM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA-binding; Methyltransferase, protein lysine, predicted; Transcription regulation; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: cytoplasm; nucleoplasm
Molecular Function: DNA binding transcription factor activity; histone deacetylase binding; metal ion binding; methyltransferase activity; promoter-specific chromatin binding; protein binding
Biological Process: adaptive immune response; artery morphogenesis; cell fate commitment; eye photoreceptor cell development; germ cell development; heart valve development; innate immune response; maternal placenta development; methylation; morphogenesis of a branching structure; negative regulation of B cell proliferation; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of B cell differentiation; positive regulation of gene expression; post-embryonic development; regulation of extrathymic T cell differentiation; regulation of natural killer cell differentiation; regulation of NK T cell differentiation; sebum secreting cell proliferation; transcription, DNA-dependent; ventricular septum development
Research Articles on PRDM1
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Product Notes
The Human PRDM1 prdm1 (Catalog #AAA280265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280265 ELISA Kit recognizes Human PRDM1. It is sometimes possible for the material contained within the vial of "PR domain zinc finger protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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