Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) Recombinant Protein | PPP1R12A recombinant protein

Recombinant Human Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) , partial

• Gene Names: PPP1R12A; MBS; M130; MYPT1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein phosphatase 1 regulatory subunit 12A (PPP1R12A); Recombinant Human Protein phosphatase 1 regulatory subunit 12A (PPP1R12A); partial; PPP1R12A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 943

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for PPP1R12A recombinant protein

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 219842212
• NCBI GeneID: 4659
• NCBI Accession #: NP_001137357.1
• NCBI GenBank Nucleotide #: NM_001143885.1
• UniProt Accession #: O14974; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0; B4DZ09; F8VWB4
• Molecular Weight: 105,641 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A isoform a
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBS; M130; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: Myosin phosphatase target subunit 1

NCBI Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.