Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit CK-1 alpha Polyclonal Antibody | anti-CK-1 alpha antibody
CK-1 alpha (Phospho-Tyr294) Antibody
Gene Names
CSNK1A1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
CK-1 alpha; Polyclonal Antibody; CK-1 alpha (Phospho-Tyr294) Antibody; CK1-alpha; CKI-alpha; CSNK1A1; Casein kinase I; alpha isoform; KC1A; kinase CK1-alpha; anti-CK-1 alpha antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
CK-1 alpha (Phospho-Tyr294) Antibody detects endogenous levels of CK-1 alpha only when phosphorylated at Tyr294.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
325
Applicable Applications for anti-CK-1 alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
IHC: 1:50~1:100
ELISA: 1:10000
Target Modification
Phospho
Modification Sites
H:Y294; M:Y294; R:Y294
Immunogen
The antiserum was produced against synthesized peptide derived from human CK-1 alpha around the phosphorylation site of Tyr294.
Immunogen Range
260-309
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kDa
NCBI Official Full Name
casein kinase I isoform alpha isoform 1
NCBI Official Synonym Full Names
casein kinase 1 alpha 1
NCBI Official Symbol
CSNK1A1
NCBI Official Synonym Symbols
CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
NCBI Protein Information
casein kinase I isoform alpha
UniProt Protein Name
Casein kinase I isoform alpha
UniProt Gene Name
CSNK1A1
UniProt Synonym Gene Names
CKI-alpha
Uniprot Description
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).
Research Articles on CK-1 alpha
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Product Notes
The CK-1 alpha csnk1a1 (Catalog #AAA9503245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CK-1 alpha (Phospho-Tyr294) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CK-1 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the CK-1 alpha csnk1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK-1 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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