Human, Mouse PPP1R12A-S507 Antibody Pair Kit | PPP1R12A antibody pair kit

Phospho-PPP1R12A-S507 Antibody Kit

Cat. Num. AAA9135717

• Gene Names: PPP1R12A; MBS; M130; MYPT1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: PPP1R12A-S507; Polyclonal Antibody Pair Kit; Phospho-PPP1R12A-S507 Antibody Kit; M130; MBS; MYPT1; PPP1R12A antibody pair kit

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 1030

• Applicable Applications for PPP1R12A antibody pair kit: Western Blot (WB)
• Immunogen: A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).; Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
• Immunogen Sequence: LASTS
• Cellular Location: Cytoplasm
• Preparation and Storage: Store at-20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot-Phospho-PPP1R12A-S507 pAb)

Western Blot (WB)

(Western blot-PPP1R12A Polyclonal Antibody)

Immunoprecipitation (IP)

(Immunoprecipitation-PPP1R12A Polyclonal Antibody)

Related Product Information for PPP1R12A antibody pair kit

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for PPP1R12A antibody pair kit

Phosphorylation pAb

NCBI and Uniprot Product Information

• NCBI GI #: 219842212
• NCBI GeneID: 4659
• NCBI Accession #: NP_001137357.1
• NCBI GenBank Nucleotide #: NM_001143885.1
• UniProt Accession #: O14974; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0; B4DZ09; F8VWB4
• Molecular Weight: Calculated MW: 105kDa; 109kDa; 111kDa; 115kDa; Observed MW: 135kDa
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A isoform a
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBS; M130; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: Myosin phosphatase target subunit 1

NCBI Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.