Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit | Ppargc1a elisa kit

Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit

Cat. Num. AAA762203

• Gene Names: PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
• Reactivity: Rat
• Synonyms: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; Ppargc1a/PGC-1-alpha/PPAR-gamma coactivator 1-alpha/PPARGC-1-alpha/Ligand effect modulator 6/LEM6/PGC1/PGC1A/PPARGC1/PGC-1v; Ppargc1a elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ppargc1a. No significant crossreactivity or interference between Ppargc1a and analogues was observed.
• Sequence Length: 798

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ppargc1a elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7019499
• NCBI GeneID: 10891
• NCBI Accession #: NP_037393.1
• NCBI GenBank Nucleotide #: NM_013261.3
• Molecular Weight: 77,139 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• NCBI Official Synonym Full Names: PPARG coactivator 1 alpha
• NCBI Official Symbol: PPARGC1A
• NCBI Official Synonym Symbols: LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Protein Family: Peroxisome proliferator-activated receptor gamma coactivator
• UniProt Gene Name: PPARGC1A
• UniProt Synonym Gene Names: LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
• UniProt Entry Name: PRGC1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Uniprot Description

PGC-1 alpha: a transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor genes. Interacts with, and regulates the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). Regulates many genes involved in energy metabolism. Provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. May be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Its transcription is repressed by ZNF746 which binds to 'insulin response sequences' in its promoter. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p15.1

Cellular Component: cell soma; DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus; PML body

Molecular Function: alpha-tubulin binding; androgen receptor binding; chromatin DNA binding; DNA binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nucleotide binding; peroxisome proliferator activated receptor binding; protein binding; RNA binding; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: aging; androgen metabolic process; androgen receptor signaling pathway; brown fat cell differentiation; cell glucose homeostasis; cellular respiration; cerebellum development; circadian regulation of gene expression; circadian rhythm; digestion; fatty acid oxidation; flavone metabolic process; forebrain development; galactose metabolic process; gluconeogenesis; mitochondrion degradation; mitochondrion organization and biogenesis; mRNA processing; negative regulation of glycolysis; negative regulation of neuron apoptosis; negative regulation of protein amino acid phosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; organelle organization and biogenesis; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of histone acetylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by pheromones; positive regulation of transcription, DNA-dependent; protein complex assembly; protein stabilization; regulation of circadian rhythm; regulation of transcription, DNA-dependent; response to cold; response to dietary excess; response to electrical stimulus involved in regulation of muscle adaptation; response to leucine; response to muscle activity; response to reactive oxygen species; response to starvation; RNA splicing; skeletal muscle atrophy; thermoregulation; transcription initiation from RNA polymerase II promoter