PPARgamma (LBD) Recombinant Protein | PPARG recombinant protein

PPARgamma (LBD) Human Recombinant (His-tagged)

Cat. Num. AAA845572

• Gene Names: PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
• Applications: SEC analysis
• Purity: >=90%
• Synonyms: PPARgamma (LBD); PPARgamma (LBD) Human Recombinant (His-tagged); PPAR-gamma; Peroxisome Proliferator-activated Receptor gamma Ligand Binding Domain; PPAR-gamma (LBD); NR1C3; PPARG (LBD); PPARG recombinant protein

• Host: E Coli; Source: Human
• Purity/Purification: >=90%
• Form/Format: Liquid; In 50 mM Tris; 100 mM NaCl; 1 mM TCEP; pH 8.0 containing 20% glycerol
• Concentration: 2 mg/ml (varies by lot)
• Sequence Length: 477

• Applicable Applications for PPARG recombinant protein: SEC analysis
• Tag Information: N-terminal His6- tag
• Reconstitution: It can be further diluted to 0.5 mg/ml with 50 mM Tris; 100 mM NaCl; 1 mM TCEP; pH 8.0 containing 20% glycerol and stored at-80 degree C in aliquots. Avoid repeated freezing and thawing cycles.
• Preparation and Storage: Store at -80 degree C for 6 months.; Centrifuge the vial prior to opening.

Testing Data

Related Product Information for PPARG recombinant protein

The peroxisome proliferator activated receptor (PPAR) family of ligand-activated transcription factors consists of three subtypes encoded by separate genes: PPARalpha; PPARdelta and PPARgamma. Of these; PPARgamma plays an important role in the regulation of fatty acid storage and glucose metabolism. The genes activated by PPARgamma stimulate lipid uptake and adipogenesis by fat cells. Many naturally occurring molecules such as; polyunsaturated fatty acids like arachidonic acid and arachidonic acid metabolites; are known to bind and activate PPARgamma. The binding of activating ligands to the ligand binding domain (LBD) of PPARgamma promotes its heterodimerization with retinoic acid-like receptor (RXR); which results in the regulated expression of target genes involved in lipid metabolism. His-PPARgamma (LBD) is a non-glycosylated protein expressed and purified from E Coli. It has been tested for functionality by its binding and subsequent displacement of 1; 8-ANS assay.

NCBI and Uniprot Product Information

• NCBI GI #: 116284370
• NCBI GeneID: 5468
• NCBI Accession #: NP_619726.2
• NCBI GenBank Nucleotide #: NM_138712.3
• UniProt Accession #: P37231; O00684; O00710; O14515; Q0QJH8; Q15178; Q15179; Q15180; Q15832; Q86U60; A8K3G6; B5BUA1
• Molecular Weight: 34.8 kDa (193-477 aa)
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma isoform 1
• NCBI Official Synonym Full Names: peroxisome proliferator activated receptor gamma
• NCBI Official Symbol: PPARG
• NCBI Official Synonym Symbols: GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma

NCBI Description

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex

Molecular Function: alpha-actinin binding; arachidonic acid binding; chromatin binding; DBD domain binding; DNA binding; double-stranded DNA binding; drug binding; enzyme binding; identical protein binding; LBD domain binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor transcription coactivator activity; peptide binding; prostaglandin receptor activity; protein binding; protein C-terminus binding; protein heterodimerization activity; protein self-association; retinoid X receptor binding; sequence-specific DNA binding; transcription activator binding; transcription factor activity; transcription factor binding; zinc ion binding

Biological Process: caspase activation; cell fate commitment; cell maturation; cellular response to insulin stimulus; epithelial cell differentiation; glucose homeostasis; innate immune response; lipid homeostasis; lipid metabolic process; lipoprotein transport; long-chain fatty acid transport; low-density lipoprotein receptor biosynthetic process; monocyte differentiation; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; placenta development; positive regulation of DNA binding; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of blood pressure; regulation of circadian rhythm; response to lipid; response to low density lipoprotein stimulus; response to nutrient; response to retinoic acid; signal transduction; transcription initiation from RNA polymerase II promoter; white fat cell differentiation

Disease: Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent; Lipodystrophy, Familial Partial, Type 3; Obesity