Rabbit anti-Human CDH9 Polyclonal Antibody | anti-CDH9 antibody

CDH9, CT (CDH9, Cadherin-9)

Cat. Num. AAA647961

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CDH9; Polyclonal Antibody; CT (CDH9; Cadherin-9); Anti -CDH9; anti-CDH9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CDH9 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: CDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 708~737 amino acids from the C-terminal region of human CDH9.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDH9 (arrow) using rabbit polyclonal CDH9 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MCF-7 cells using CDH9 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDH9 antibody

CDH9 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins.

Product Categories/Family for anti-CDH9 antibody

Antibodies; Abs to Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 90193626
• NCBI GeneID: 1007
• NCBI Accession #: NP_057363.3
• NCBI GenBank Nucleotide #: NM_016279.3
• UniProt Accession #: Q9ULB4; Q3B7I5
• Molecular Weight: 88,689 Da
• NCBI Official Full Name: cadherin-9 preproprotein
• NCBI Official Synonym Full Names: cadherin 9, type 2 (T1-cadherin)
• NCBI Official Symbol: CDH9
• NCBI Protein Information: cadherin-9; T1-cadherin
• UniProt Protein Name: Cadherin-9
• Protein Family: Cadherin
• UniProt Gene Name: CDH9
• UniProt Entry Name: CADH9_HUMAN

NCBI Description

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Subcellular location: Cell membrane; Single-pass type I membrane protein

Potential.

Domain: Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain

By similarity.

Sequence similarities: Contains 5 cadherin domains.