Neuron Specific Enolase (NSE) Native Protein | Eno2 native protein

Neuron Specific Enolase (NSE)

Cat. Num. AAA318316

• Gene Names: Eno2; NSE; RNEN3
• Applications: ELISA, Western Blot
• Purity: >95% pure (SDS-PAGE). Ion-exchange chromatography and gel-filtration
• Synonyms: Neuron Specific Enolase (NSE); Subunit composition is gamma-gamma; Eno2 native protein

• Host: Post mortal Human Brain
• Specificity: Neuron Specific Enolase (NSE)
• Purity/Purification: >95% pure (SDS-PAGE). Ion-exchange chromatography and gel-filtration
• Form/Format: Purified, Liquid
• Concentration: 8.4mg/ml (BCA with SDS) (varies by lot)

• Applicable Applications for Eno2 native protein: EIA/ELISA, Western Blot
• Inactivation: Not applicable
• Buffer: 0.1M Tris-HCl, 5mM Mg2SO4, 0.25M KCl, pH 5.1
• Preservative: No
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Warnings: Blood sample from tissue donor tested negative for HIV 1, HIV 2, HCV antibodies, HBsAg and Syphilis. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentia
• Preparation and Storage: Store at -20 degree C. Avoid multiple freeze/thaw cycles.

Product Categories/Family for Eno2 native protein

Purified Neuroscience Reagents

References

• J. Biochem., (1980), 87, 1587-1594.
• Pahlman S., et al. (1984), Tumour Biology, v.5, pp. 127-139.

NCBI and Uniprot Product Information

• NCBI GI #: 2465396
• NCBI GeneID: 24334
• UniProt Accession #: P07323
• Molecular Weight: 47,141 Da
• NCBI Official Full Name: neuron-specific enolase
• NCBI Official Synonym Full Names: enolase 2, gamma, neuronal
• NCBI Official Symbol: Eno2
• NCBI Official Synonym Symbols: NSE; RNEN3
• NCBI Protein Information: gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Gamma-enolase
• Protein Family: Enolase
• UniProt Gene Name: Eno2
• UniProt Synonym Gene Names: Eno-2
• UniProt Entry Name: ENOG_RAT

NCBI Description

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD]

Uniprot Description

Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Ref.6

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form

By similarity. Ref.7

Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. ENO2 levels in brain increase 10-30 days after birth. Levels continue to accumulate over the following few months (protein only).

Sequence similarities: Belongs to the enolase family.