Porcine Osteopontin (OPN) RTU ELISA Kit | OPN rtu elisa kit

Porcine Osteopontin (OPN) ELISA Kit

Cat. Num. AAA4501584

• Gene Names: SPP1; Opn
• Reactivity: Porcine
• Synonyms: Osteopontin (OPN); Porcine Osteopontin (OPN) ELISA Kit; SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin; OPN rtu elisa kit

• Reactivity: Porcine
• Sequence Length: 303

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 29.8pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPN rtu elisa kit

Test Principle: antibody specific to OPN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47522868
• NCBI GeneID: 397087
• NCBI Accession #: NP_999188.1
• NCBI GenBank Nucleotide #: NM_214023.1
• UniProt Accession #: P14287
• Molecular Weight: 33,669 Da
• NCBI Official Full Name: osteopontin
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: Opn
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: OPN; SPP-1

Uniprot Description

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.