Rabbit CAPN1 Polyclonal Antibody | anti-CAPN1 antibody

CAPN1 Antibody

Cat. Num. AAA9406489

• Gene Names: CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CAPN1; Polyclonal Antibody; CAPN1 Antibody; CANP; CANP1; CANPL1; muCANP; anti-CAPN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CAPN1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 714

• Applicable Applications for anti-CAPN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human CAPN1.
• Target Name: CAPN1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CAPN1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney using CAPN1 antibody at dilution of 1:200 (400x lens).)

Related Product Information for anti-CAPN1 antibody

Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).

Product Categories/Family for anti-CAPN1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 311893363
• NCBI GeneID: 823
• NCBI Accession #: NP_001185797.1
• NCBI GenBank Nucleotide #: NM_001198868.1
• UniProt Accession #: P07384; Q2TTR0; Q6DHV4
• Molecular Weight: 81,890 Da
• NCBI Official Full Name: calpain-1 catalytic subunit
• NCBI Official Synonym Full Names: calpain 1, (mu/I) large subunit
• NCBI Official Symbol: CAPN1
• NCBI Official Synonym Symbols: CANP; muCL; CANP1; CANPL1; muCANP
• NCBI Protein Information: calpain-1 catalytic subunit
• UniProt Protein Name: Calpain-1 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: CAPN1
• UniProt Synonym Gene Names: CANPL1; CANP 1; muCANP
• UniProt Entry Name: CAN1_HUMAN

NCBI Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family.

Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.22.52

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: focal adhesion; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; receptor catabolic process; positive regulation of cell proliferation; proteolysis