Porcine Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) ELISA Kit | VEGFR2 elisa kit

Porcine Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) ELISA Kit

Cat. Num. AAA456488

• Gene Names: KDR; FLK-1
• Reactivity: Porcine
• Synonyms: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); Porcine Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) ELISA Kit; VEGFR2 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGFR2. No significant cross-reactivity or interference between VEGFR2 and analogues was observed.
• Sequence Length: 1,356

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.62ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFR2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFR2 in porcine serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VEGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 350587519
• NCBI GeneID: 397311
• NCBI Accession #: XP_003129035.3
• NCBI GenBank Nucleotide #: XP_003129035.3
• UniProt Accession #: K7GNF0
• Molecular Weight: 151,580 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 2 isoform X1
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK-1
• NCBI Protein Information: kinase insert domain receptor; flk-1 type VEGF receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); vascular endothelial growth factor receptor 2
• UniProt Protein Name: Uncharacterized protein
• UniProt Gene Name: KDR