Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit | RAMP2 elisa kit

Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit

Cat. Num. AAA2019044

• Reactivity: Human
• Synonyms: Receptor Activity Modifying Protein 2 (RAMP2); Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit; Calcitonin-receptor-like receptor activity-modifying protein 2; Receptor(G Protein-Coupled)Activity Modifying Protein 2; Receptor(Calcitonin)Activity Modifying Protein 2; RAMP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAMP2. No significant cross-reactivity or interference between RAMP2 and analogues was observed.
• Sequence Length: 175

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.071ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAMP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAMP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAMP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RAMP2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to RAMP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RAMP2 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 118572585
• NCBI GeneID: 10266
• NCBI Accession #: NP_005845.2
• NCBI GenBank Nucleotide #: NM_005854.2
• UniProt Accession #: O60895; Q8N1F2; A7L9S6; K7EMD3
• Molecular Weight: 20,063 Da
• NCBI Official Full Name: receptor activity-modifying protein 2
• NCBI Official Synonym Full Names: receptor (G protein-coupled) activity modifying protein 2
• NCBI Official Symbol: RAMP2
• NCBI Protein Information: receptor activity-modifying protein 2
• UniProt Protein Name: Receptor activity-modifying protein 2
• Protein Family: Receptor activity-modifying protein
• UniProt Gene Name: RAMP2
• UniProt Synonym Gene Names: CRLR activity-modifying protein 2
• UniProt Entry Name: RAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq, Jul 2008]

Uniprot Description

RAMP2: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Belongs to the RAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q12-q21.1

Cellular Component: cell surface; lysosome; integral to plasma membrane; cytoplasm; plasma membrane; coated pit; receptor complex

Molecular Function: protein binding; coreceptor activity; protein transporter activity

Biological Process: heart development; female pregnancy; response to estradiol stimulus; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; intracellular protein transport; protein transport; positive regulation of angiogenesis; receptor internalization; calcium ion transport; regulation of blood pressure; positive regulation of cAMP biosynthetic process; negative regulation of vascular permeability; response to hypoxia; cAMP biosynthetic process; sprouting angiogenesis; angiogenesis; response to progesterone stimulus; vasculogenesis; regulation of G-protein coupled receptor protein signaling pathway