Porcine SYP ELISA Kit | SYP elisa kit
Porcine SYP (Synaptophysin) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SYP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SYP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SYP. You can calculate the concentration of SYP in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Vesicle
Cellular Component: neuron projection; protein complex; synaptic vesicle membrane; integral to membrane; terminal button; intracellular organelle; excitatory synapse; integral to synaptic vesicle membrane; presynaptic membrane; synaptic vesicle; membrane; presynaptic active zone; synapse; cytoplasmic vesicle; cell junction
Molecular Function: protein domain specific binding; identical protein binding; protein binding; transporter activity; protein complex binding; cholesterol binding; syntaxin-1 binding; SH2 domain binding
Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic transmission; transport; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity
Research Articles on SYP
Similar Products
Product Notes
The Porcine SYP syp (Catalog #AAA2509285) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509285 ELISA Kit recognizes Porcine SYP. It is sometimes possible for the material contained within the vial of "SYP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
