Rabbit anti-Mouse Frk Polyclonal Antibody | anti-Frk antibody

Frk, CT (Frk, Bsk, Iyk, Tyrosine-protein kinase FRK, Beta-cell Src-homology tyrosine kinase, FYN-related kinase, Intestine tyrosine kinase) (PE)

Cat. Num. AAA6300135

• Gene Names: Frk; BSK; GTK; RAK; C85044; BSK/IYK
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Frk; Polyclonal Antibody; CT (Frk; Bsk; Iyk; Tyrosine-protein kinase FRK; Beta-cell Src-homology tyrosine kinase; FYN-related kinase; Intestine tyrosine kinase) (PE); Intestine tyrosine kinase; anti-Frk antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 512

• Applicable Applications for anti-Frk antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Frk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463-491 amino acids from the C-terminal region of Mouse Frk.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Frk Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Frk antibody detected the Frk protein (arrow).)

Related Product Information for anti-Frk antibody

Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor (By similarity).

Product Categories/Family for anti-Frk antibody

Antibodies; Protein Kinases

References

Akerblom, B., et al. Mol. Cell. Endocrinol. 270 (1-2), 73-78 (2007): Nishimura, M., et al. DNA Res. 11(5):315-323(2004). Sharov, A.A., et al. PLoS Biol. 1 (3), E74 (2003): Cowen, M.S., et al. Alcohol. Clin. Exp. Res. 27(8):1213-1219(2003). Serfas, M.S., et al. Oncol. Res. 13 (6-10), 409-419 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 31542823
• NCBI GeneID: 14302
• NCBI Accession #: NP_034367.2; NP_001153016.1
• NCBI GenBank Nucleotide #: NP_034367.2
• UniProt Accession #: Q922K9
• NCBI Official Full Name: tyrosine-protein kinase FRK
• NCBI Official Synonym Full Names: fyn-related kinase
• NCBI Official Symbol: Frk
• NCBI Official Synonym Symbols: BSK; GTK; RAK; C85044; BSK/IYK
• NCBI Protein Information: tyrosine-protein kinase FRK
• UniProt Protein Name: Tyrosine-protein kinase FRK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Frk
• UniProt Synonym Gene Names: Bsk; Iyk; BSK
• UniProt Entry Name: FRK_MOUSE

Uniprot Description

FRK: a tyrosine kinase of the Src family. Expressed in cell lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. A nuclear protein that may function during G1 and S phase of the cell cycle to suppress growth.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Tumor suppressor; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; nucleus

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; receptor binding

Biological Process: cell migration; innate immune response; cell differentiation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation