Typical Testing Data/Standard Curve (for reference only)
Porcine Growth Hormone Releasing Hormone (GHRH) ELISA Kit | GHRH elisa kit
Pig Growth Hormone Releasing Hormone (GHRH) ELISA Kit
Gene Names
GHRHR; GRFR; GHRFR; IGHD1B
Reactivity
Porcine
Synonyms
Growth Hormone Releasing Hormone (GHRH); Pig Growth Hormone Releasing Hormone (GHRH) ELISA Kit; GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin; GHRH elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH).
No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
Sequence Length
235
Assay Type
Competitive Inhibition
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
12.35-1000pg/mL
Sensitivity
< 5.22pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GHRH elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone Releasing Hormone (GHRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone Releasing Hormone (GHRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,402 Da
NCBI Official Full Name
growth hormone releasing hormone, partial
NCBI Official Synonym Full Names
growth hormone releasing hormone receptor
NCBI Official Symbol
GHRHR
NCBI Official Synonym Symbols
GRFR; GHRFR; IGHD1B
NCBI Protein Information
growth hormone-releasing hormone receptor
UniProt Protein Name
Growth hormone-releasing hormone receptor
Protein Family
UniProt Gene Name
GHRHR
UniProt Synonym Gene Names
GHRH receptor; GRF receptor; GRFR
NCBI Description
This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]
Uniprot Description
Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
Research Articles on GHRH
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Product Notes
The Porcine GHRH ghrhr (Catalog #AAA2700117) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2700117 ELISA Kit recognizes Porcine GHRH. It is sometimes possible for the material contained within the vial of "Growth Hormone Releasing Hormone (GHRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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