PRDX5 active protein

PRDX5, Recombinant, Human (Peroxiredoxin-5, Mitochondrial, Peroxiredoxin V, Prx-V, Peroxisomal Antioxidant Enzyme, PLP, Thioredoxin Reductase, Thioredoxin Peroxidase PMP20, Antioxidant Enzyme B166, AOEB166, TPx Type VI, Liver Tissue 2D-page Spot 71B, Alu

Cat. Num. AAA636327

• Gene Names: PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: PRDX5; Recombinant; Human (Peroxiredoxin-5; Mitochondrial; Peroxiredoxin V; Prx-V; Peroxisomal Antioxidant Enzyme; PLP; Thioredoxin Reductase; Thioredoxin Peroxidase PMP20; Antioxidant Enzyme B166; AOEB166; TPx Type VI; Liver Tissue 2D-page Spot 71B; Alu; PRDX5 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from 20mM HEPES, pH 7.4. Reconstitute with sterile dH2O.

• Specific Activity: 2.12U/mg
• Unit Definition: One unit will cause the oxidation of 1umole of NADPH at 25 degree C at pH 7.5
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

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Related Product Information for PRDX5 active protein

Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the aa sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30kD in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.

Recombinant corresponding to aa53-214 without mitochondrial leader sequence, from human Peroxiredoxin V, expressed in E. coli.

Product Categories/Family for PRDX5 active protein

Molecular Biology; MB-Enzymes; Peroxiredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 32455262
• NCBI GeneID: 25824
• NCBI Accession #: NP_857635.1
• NCBI GenBank Nucleotide #: NM_181652.2
• UniProt Accession #: P30044; Q14CK0; Q6IAF2; Q9UBU5; Q9UJU4; Q9UKX4; A6NC19; A6NG06; B7ZLJ4; B7ZVW3
• Molecular Weight: ~17kD
• NCBI Official Full Name: peroxiredoxin-5, mitochondrial isoform c
• NCBI Official Synonym Full Names: peroxiredoxin 5
• NCBI Official Symbol: PRDX5
• NCBI Official Synonym Symbols: PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166
• NCBI Protein Information: peroxiredoxin-5, mitochondrial; TPx type VI; peroxiredoxin V; Alu co-repressor 1; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; liver tissue 2D-page spot 71B; peroxisomal antioxidant enzyme
• UniProt Protein Name: Peroxiredoxin-5, mitochondrial
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX5
• UniProt Synonym Gene Names: ACR1; AOEB166; Prx-V
• UniProt Entry Name: PRDX5_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: peroxisomal matrix; extracellular space; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; negative regulation of transcription from RNA polymerase III promoter; negative regulation of oxidoreductase activity; positive regulation of collagen biosynthetic process; response to oxidative stress; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis