Mouse Nestin Monoclonal Antibody | anti-NES antibody

Anti-Nestin

Cat. Num. AAA500041

• Gene Names: NES; Nbla00170
• Applications: Western Blot, Immunofluorescence
• Purity: Protein G purified culture supernatant
• Synonyms: Nestin; Monoclonal Antibody; Anti-Nestin; anti-NES antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG₁
• Clone Number: 4D11
• Specificity: Specific for the ~220-240 kDa nestin doublet in Western blots of rat brain homogenate.
• Purity/Purification: Protein G purified culture supernatant
• Form/Format: 100ul in PBS + 50% Glycerol + 5 mM NaN₃. Adequate amount of material to conduct 10 mini Western blots.

• Applicable Applications for anti-NES antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:2,000; IF: 1:500
• Quality Control Tests: Western blots performed on each lot.
• Species Tested: Human, Mouse, Rat
• Immunogen: Recombinant human Nestin expressed in and purifeid from E. coli
• Biological Significance: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, calledRat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.
• Preparation and Storage: Store at -20°C; stable for at least one year.

Western Blot (WB)

Immunostaining

(Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (MBS500041, red, 1:500) and anti-MAP2 antibody (MBS502140, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.)

References

• Lendahl U, Zimmerman LB and McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell 60:585-95 (1990).
• Hockfield S and McKay RD. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5:3310-3328 (1985).

NCBI and Uniprot Product Information

• NCBI GI #: 38176300
• NCBI GeneID: 10763
• NCBI Accession #: NP_006608.1
• NCBI GenBank Nucleotide #: NM_006617.1
• UniProt Accession #: P48681; O00552; Q3LIF5; Q5SYZ6
• Molecular Weight: ~220-240 kDa
• NCBI Official Full Name: nestin
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• Protein Family: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells

By similarity.

Subunit structure: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

By similarity.

Tissue specificity: CNS stem cells.

Developmental stage: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Post-translational modification: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized

By similarity.

Sequence similarities: Belongs to the intermediate filament family.