Porcine Glycated albumin (GA) ELISA Kit | GA elisa kit

Porcine Glycated albumin (GA) ELISA Kit

Cat. Num. AAA261330

• Gene Names: GLS2; GA; GLS; LGA; hLGA
• Reactivity: Porcine
• Synonyms: Glycated albumin (GA); Porcine Glycated albumin (GA) ELISA Kit; GA elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 337

• Samples: Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant GA concentration
• Assay Type: Sandwich
• Detection Range: 1000 ng/ml-15.6 ng/ml
• Sensitivity: 5 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GA elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine GA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 526118241
• NCBI GeneID: 27165
• NCBI Accession #: NP_001267726.1
• NCBI GenBank Nucleotide #: NM_001280797.1
• Molecular Weight: 66,323 Da
• NCBI Official Full Name: glutaminase liver isoform, mitochondrial isoform 4
• NCBI Official Synonym Full Names: glutaminase 2 (liver, mitochondrial)
• NCBI Official Symbol: GLS2
• NCBI Official Synonym Symbols: GA; GLS; LGA; hLGA
• NCBI Protein Information: glutaminase liver isoform, mitochondrial; glutaminase I; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase
• UniProt Protein Name: Glutaminase liver isoform, mitochondrial
• UniProt Gene Name: GLS2
• UniProt Synonym Gene Names: GA; GLS
• UniProt Entry Name: GLSL_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.

Protein type: Hydrolase; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; EC 3.5.1.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; glutaminase activity

Biological Process: amino acid metabolic process; regulation of apoptosis; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process