Porcine GCK ELISA Kit | GCK elisa kit
Porcine GCK (Glucokinase) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GCK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GCK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GCK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GCK. You can calculate the concentration of GCK in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]
Uniprot Description
GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a ""glucose sensor"" in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose; Kinase, other; EC 2.7.1.2
Chromosomal Location of Human Ortholog: 7p15.3-p15.1
Cellular Component: nucleoplasm; mitochondrion; actin filament; cytosol; basal cortex; secretory granule
Molecular Function: protein binding; mannokinase activity; magnesium ion binding; fructokinase activity; ADP binding; glucokinase activity; protein phosphatase binding; ATP binding; glucose binding
Biological Process: glucose 6-phosphate metabolic process; positive regulation of glycogen biosynthetic process; lipid homeostasis; pathogenesis; glucose transport; glucose homeostasis; NADP metabolic process; cellular response to glucose starvation; elevation of cytosolic calcium ion concentration; detection of glucose; regulation of glycolysis; negative regulation of gluconeogenesis; transmembrane transport; second-messenger-mediated signaling; glycogen biosynthetic process; glycolysis; regulation of potassium ion transport; positive regulation of insulin secretion; glucose metabolic process; endocrine pancreas development; fructose 2,6-bisphosphate metabolic process; cellular response to insulin stimulus; hexose transport; carbohydrate metabolic process; positive regulation of glycolysis; carbohydrate phosphorylation; negative regulation of epinephrine secretion; cell glucose homeostasis; regulation of insulin secretion; positive regulation of phosphorylation
Disease: Hyperinsulinemic Hypoglycemia, Familial, 3; Diabetes Mellitus, Permanent Neonatal; Maturity-onset Diabetes Of The Young, Type 2; Diabetes Mellitus, Noninsulin-dependent
Research Articles on GCK
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Product Notes
The Porcine GCK gck (Catalog #AAA2512663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512663 ELISA Kit recognizes Porcine GCK. It is sometimes possible for the material contained within the vial of "GCK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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