Porcine Cluster Of Differentiation 14 (CD14) ELISA Kit | CD14 elisa kit

Porcine Cluster Of Differentiation 14 (CD14) ELISA Kit

Cat. Num. AAA455225

• Reactivity: Porcine
• Synonyms: Cluster Of Differentiation 14 (CD14); Porcine Cluster Of Differentiation 14 (CD14) ELISA Kit; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD14. No significant cross-reactivity or interference between CD14 and analogues was observed.
• Sequence Length: 373

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 6.25-400ng/mL
• Sensitivity: 2.15ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD14 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD14 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD14 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD14 in porcine serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156071434
• NCBI GeneID: 100037938
• NCBI Accession #: NP_001090914.2
• NCBI GenBank Nucleotide #: NM_001097445.2
• UniProt Accession #: A7BG66
• Molecular Weight: 39,724 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14

Uniprot Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-.