Porcine CLDN3 (Claudin-3) ELISA Kit | CLDN3 elisa kit

Porcine CLDN3 (Claudin-3) ELISA Kit

Cat. Num. AAA7606132

• Gene Names: CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• Reactivity: Porcine
• Synonyms: CLDN3 (Claudin-3); Porcine CLDN3 (Claudin-3) ELISA Kit; CLDN3 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLDN3. No significant cross-reactivity or interference between CLDN3 and analogues was observed.
• Sequence Length: 220

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CLDN3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502875
• NCBI GeneID: 1365
• NCBI Accession #: NP_001297.1
• NCBI GenBank Nucleotide #: NM_001306.3
• UniProt Accession #: O15551
• Molecular Weight: 23,319 Da
• NCBI Official Full Name: claudin-3
• NCBI Official Synonym Full Names: claudin 3
• NCBI Official Symbol: CLDN3
• NCBI Official Synonym Symbols: RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• NCBI Protein Information: claudin-3
• UniProt Protein Name: Claudin-3
• Protein Family: Claudin
• UniProt Gene Name: CLDN3
• UniProt Synonym Gene Names: C7orf1; CPETR2; CPE-R 2; CPE-receptor 2; hRVP1

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family.

Protein type: Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: integral to membrane; integral to plasma membrane; tight junction

Molecular Function: transmembrane receptor activity

Biological Process: protein heterooligomerization; protein homooligomerization; response to hypoxia