Human Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) RTU ELISA Kit | P2RX7 rtu elisa kit

Human Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) ELISA Kit

Cat. Num. AAA4501618

• Gene Names: P2RX7; P2X7
• Reactivity: Human
• Synonyms: Purinergic Receptor P2X; Ligand Gated Ion Channel 7 (P2RX7); Human Purinergic Receptor P2X; Ligand Gated Ion Channel 7 (P2RX7) ELISA Kit; P2X7; P2X Purinoceptor 7; ATP receptor; Purinergic receptor; P2RX7 rtu elisa kit

• Reactivity: Human
• Sequence Length: 595

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.112ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for P2RX7 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P2RX7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to P2RX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P2RX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P2RX7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300068987
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.3
• NCBI GenBank Nucleotide #: NM_002562.5
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: 58,239 Da
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7
• UniProt Protein Name: P2X purinoceptor 7
• Protein Family: P2X purinoceptor
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Uniprot Description

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).