Porcine Caspase-9 ELISA Kit | Caspase-9 elisa kit

Porcine Caspase-9 ELISA Kit

Cat. Num. AAA736852

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Porcine
• Synonyms: Caspase-9; Porcine Caspase-9 ELISA Kit; Caspase-9 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Caspase-9 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This Caspase-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Caspase-9. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY Caspase-9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Caspase-9. Standards or samples are then added to the microtiter plate wells and Caspase-9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Caspase-9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Caspase-9 are added to each well to “sandwich” the Caspase-9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Caspase-9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caspase-9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Caspase-9 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 6518502
• NCBI GeneID: 842
• Molecular Weight: 36,564 Da
• NCBI Official Full Name: caspase-9
• NCBI Official Synonym Full Names: caspase 9, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Protease; Apoptosis; EC 3.4.22.62

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: mitochondrion; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; SH3 domain binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; caspase activation via cytochrome c; platelet formation; response to DNA damage stimulus; aging