Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit | FABP3 elisa kit

Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

Cat. Num. AAA2703066

• Gene Names: FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
• Reactivity: Human
• Synonyms: Fatty Acid Binding Protein 3; Muscle And Heart (FABP3); Human Fatty Acid Binding Protein 3; Muscle And Heart (FABP3) ELISA Kit; MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein; FABP3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 3, Muscle And Heart (FABP3).; No significant cross-reactivity or interference between Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) and analogues was observed.
• Sequence Length: 133

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.251ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 3, Muscle And Heart (FABP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 3, Muscle And Heart (FABP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 3, Muscle And Heart (FABP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119628024
• NCBI GeneID: 2170
• UniProt Accession #: P05413; Q5VV93; Q99957; B2RAB6
• Molecular Weight: 14,858 Da
• NCBI Official Full Name: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor), isoform CRA_b
• NCBI Official Synonym Full Names: fatty acid binding protein 3
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; M-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart
• UniProt Protein Name: Fatty acid-binding protein, heart
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI; H-FABP; MDGI; M-FABP

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.