Typical Testing Data/Standard Curve (for reference only)
Porcine ANG II ELISA Kit | ANG II elisa kit
Porcine ANG II (Angiotensin II) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine ANG II . During the reaction, Porcine ANG II in the sample or standard competes with a fixed amount of Porcine ANG II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine ANG II . Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the Substrate Reagent is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine ANG II in samples can be calculated by comparing the OD of the samples with the standard curve.
NCBI and Uniprot Product Information
NCBI Description
plays a role in regulation of blood pressure [RGD, Feb 2006]
Uniprot Description
angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cytoplasm
Molecular Function: angiotensin receptor binding; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; hormone activity; type 2 angiotensin receptor binding; type 1 angiotensin receptor binding
Biological Process: positive regulation of catalytic activity; extracellular matrix organization and biogenesis; renal system process; positive regulation of nitric oxide biosynthetic process; establishment of blood-nerve barrier; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; female pregnancy; positive regulation of multicellular organism growth; positive regulation of vasodilation; ovarian follicle rupture; regulation of apoptosis; positive regulation of fibroblast proliferation; cell surface receptor linked signal transduction; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; positive regulation of superoxide release; negative regulation of neuron apoptosis; kidney development; angiotensin mediated regulation of renal output; regulation of calcium ion transport; response to muscle activity involved in regulation of muscle adaptation; regulation of norepinephrine secretion; brain renin-angiotensin system; negative regulation of tissue remodeling; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; phospholipase C activation; regulation of gene expression; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; regulation of transmission of nerve impulse; smooth muscle cell differentiation; cytokine secretion; positive regulation of blood pressure; regulation of long-term neuronal synaptic plasticity; peristalsis; cell-matrix adhesion; positive regulation of cellular protein metabolic process; drinking behavior; renin-angiotensin regulation of aldosterone production; smooth muscle cell proliferation; excretion; vasodilation; response to salt stress; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; fibroblast proliferation; regulation of blood pressure; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; angiotensin mediated drinking behavior; artery smooth muscle contraction; aging; positive regulation of fatty acid biosynthetic process; vasoconstriction; blood vessel development; cellular sodium ion homeostasis; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; activation of NF-kappaB-inducing kinase; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; ureteric bud branching; regulation of inflammatory response; hormone metabolic process; response to cold; negative regulation of cell growth; astrocyte activation
Research Articles on ANG II
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Product Notes
The Porcine ANG II agt (Catalog #AAA2509981) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509981 ELISA Kit recognizes Porcine ANG II. It is sometimes possible for the material contained within the vial of "ANG II, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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