Angiotensinogen / SerpinA8 / AGT Recombinant Protein | AGT recombinant protein

Rat Angiotensinogen / SerpinA8 / AGT Protein (His Tag)

Cat. Num. AAA2547255

• Gene Names: Agt; Ang; PAT; ANRT; AngII
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: Angiotensinogen / SerpinA8 / AGT; Rat Angiotensinogen / SerpinA8 / AGT Protein (His Tag); Serpina8; AGT recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 477

• Application Notes: The recombinant rat Agt consists 464 amino acids and predicts a molecular mass of 51 kDa.
• Predicted N Terminal: Asp 25
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AGT recombinant protein

Background: Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des (Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.

Description: A DNA sequence encoding the rat Agt (NP_602308.1) (Met1-Val477) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 19705570
• NCBI GeneID: 24179
• NCBI Accession #: NP_602308.1
• NCBI GenBank Nucleotide #: NM_134432.2
• UniProt Accession #: P01015
• Molecular Weight: 51,982 Da
• NCBI Official Full Name: angiotensinogen
• NCBI Official Synonym Full Names: angiotensinogen
• NCBI Official Symbol: Agt
• NCBI Official Synonym Symbols: Ang; PAT; ANRT; AngII
• NCBI Protein Information: angiotensinogen
• UniProt Protein Name: Angiotensinogen
• Protein Family: Angiotensinogen
• UniProt Gene Name: Agt
• UniProt Synonym Gene Names: Serpina8; Ang I; Ang II; Ang III; Ang IV

NCBI Description

plays a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: cytoplasm; extracellular space; mitochondrion

Molecular Function: angiotensin receptor binding; hormone activity; receptor agonist activity; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding

Biological Process: activation of NF-kappaB-inducing kinase; aging; angiotensin mediated drinking behavior; angiotensin mediated regulation of renal output; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; artery smooth muscle contraction; astrocyte activation; blood vessel development; brain renin-angiotensin system; cell surface receptor linked signal transduction; cell-matrix adhesion; cellular sodium ion homeostasis; cytokine secretion; drinking behavior; elevation of cytosolic calcium ion concentration; establishment of blood-nerve barrier; excretion; extracellular matrix organization and biogenesis; female pregnancy; fibroblast proliferation; G-protein coupled receptor protein signaling pathway; hormone metabolic process; kidney development; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of nerve growth factor receptor signaling pathway; negative regulation of neuron apoptosis; negative regulation of tissue remodeling; ovarian follicle rupture; peristalsis; phospholipase C activation; positive regulation of blood pressure; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of cytokine secretion; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fatty acid biosynthetic process; positive regulation of fibroblast proliferation; positive regulation of MAPKKK cascade; positive regulation of multicellular organism growth; positive regulation of nitric oxide biosynthetic process; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of superoxide release; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of apoptosis; regulation of blood pressure; regulation of calcium ion transport; regulation of gene expression; regulation of heart rate; regulation of inflammatory response; regulation of long-term neuronal synaptic plasticity; regulation of norepinephrine secretion; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; regulation of transmission of nerve impulse; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; renal system process; renin-angiotensin regulation of aldosterone production; response to cold; response to muscle activity involved in regulation of muscle adaptation; response to salt stress; smooth muscle cell differentiation; smooth muscle cell proliferation; stress-activated MAPK cascade; ureteric bud branching; vasoconstriction; vasodilation