CBR3 recombinant protein

CBR3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121753

• Gene Names: CBR3; hCBR3; SDR21C2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CBR3; CBR3 Protein; Human; Recombinant (His Tag); Human CBR3/HCBR3/Carbonyl Reductase 3 Protein (His Tag); hCBR3 Protein; HEL-S-25 Protein; SDR21C2 Protein; carbonyl reductase 3; CBR3 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Trp277

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CBR3 (O75828)(Met1-Trp277) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CBR3 recombinant protein

Background: CBR3, also known as hCBR3, belongs to the short-chain dehydrogenases/reductases (SDR) family. CBR3 is expressed in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis. It is a monomeric NADPH-dependent oxidoreductase and is closely linked to another carbonyl reductase gene-CBR1. CBR3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

References

Lakhman SS. et al., 2005. Drug Metab Dispos. 33 (2): 254-7. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.

NCBI and Uniprot Product Information

• NCBI GI #: 4502601
• NCBI GeneID: 874
• NCBI Accession #: NP_001227.1
• NCBI GenBank Nucleotide #: NM_001236.3
• UniProt Accession #: O75828; Q6FHP2
• Molecular Weight: 30,850 Da
• NCBI Official Full Name: carbonyl reductase
• NCBI Official Synonym Full Names: carbonyl reductase 3
• NCBI Official Symbol: CBR3
• NCBI Official Synonym Symbols: hCBR3; SDR21C2
• NCBI Protein Information: carbonyl reductase [NADPH] 3; carbonyl reductase [NADPH] 3; carbonyl reductase (NADPH) 3; NADPH-dependent carbonyl reductase 3; short chain dehydrogenase/reductase family 21C, member 2
• UniProt Protein Name: Carbonyl reductase [NADPH] 3
• Protein Family: Carbonyl reductase
• UniProt Gene Name: CBR3
• UniProt Entry Name: CBR3_HUMAN

NCBI Description

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008]

Uniprot Description

CBR3: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; EC 1.1.1.184; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol

Molecular Function: 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity

Biological Process: phylloquinone catabolic process; cognition