Typical Testing Data/Standard Curve (for reference only)
Porcine Allograft inflammatory factor 1-like (AIF1L) ELISA Kit | AIF1L elisa kit
Porcine Allograft inflammatory factor 1-like (AIF1L) ELISA Kit
Gene Names
Aif1l; Iba2; C87647; AI043124; 2810003C17Rik
Reactivity
Porcine
Synonyms
Allograft inflammatory factor 1-like (AIF1L); Porcine Allograft inflammatory factor 1-like (AIF1L) ELISA Kit; AIF1L elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This AIF1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AIF1L. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AIF1L elisa kit
Principle of the assay: AIF1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AIF1L antibody and an AIF1L-HRP conjugate. The assay sample and buffer are incubated together with AIF1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIF1L concentration since AIF1L from samples and AIF1L-HRP conjugate compete for the anti-AIF1L antibody binding site. Since the number of sites is limited, as more sites are occupied by AIF1L from the sample, fewer sites are left to bind AIF1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIF1L concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AIF1L elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,024 Da
NCBI Official Full Name
allograft inflammatory factor 1-like
NCBI Official Synonym Full Names
allograft inflammatory factor 1-like
NCBI Official Symbol
Aif1l
NCBI Official Synonym Symbols
Iba2; C87647; AI043124; 2810003C17Rik
NCBI Protein Information
allograft inflammatory factor 1-like; ionized calcium binding adapter molecule 2; ionized calcium-binding adapter molecule 2
UniProt Protein Name
Allograft inflammatory factor 1-like
Protein Family
UniProt Gene Name
Aif1l
UniProt Synonym Gene Names
Iba2
UniProt Entry Name
AIF1L_MOUSE
Uniprot Description
IBA2: Actin-binding protein that promotes actin bundling. May neither bind calcium nor depend on calcium for function. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Calcium-binding
Cellular Component: cytoskeleton; protein complex; cell projection; focal adhesion; membrane; cytoplasm; plasma membrane; actin filament; actin cytoskeleton
Molecular Function: actin filament binding; metal ion binding; calcium ion binding; actin binding
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Product Notes
The Porcine AIF1L aif1l (Catalog #AAA7224287) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7224287 ELISA Kit recognizes Porcine AIF1L. It is sometimes possible for the material contained within the vial of "Allograft inflammatory factor 1-like (AIF1L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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