Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat transglutaminase 4 Polyclonal Antibody | anti-TGM4 antibody
Goat anti-transglutaminase 4 (aa49-63) Antibody
Gene Names
TGM4; TGP; hTGP
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
transglutaminase 4; Polyclonal Antibody; Goat anti-transglutaminase 4 (aa49-63) Antibody; TGM4; transglutaminase 4 (prostate); FLJ26776; TGP; hTGP; TG(P); TGase P; TGase-4; fibrinoligase; prostate transglutaminase; prostate-specific transglutaminase; protein-glutamine gamma-glutamyltransferase 4; transglutaminase P; transglutaminase-4; fibrinoligase antibody; FLJ26776 antibody; hTGP antibody; prostate transglutaminase antibody; prostate-specific transglutaminase antibody; protein-glutamine gamma-glutamyltransferase 4 antibody; TG(P) antibody; TGase P antibody; TGase-4 antibody; TGP antibody; transglutaminase 4 (prostate) antibody; transglutaminase P antibody; transglutaminase-4 antibody; TGM4 antibody; transglutaminase 4 (aa49-63); anti-TGM4 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NQPLQSYHQLKLEFS
Sequence Length
684
Applicable Applications for anti-TGM4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 77.1kDa according to NP_003232.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 77.1kDa according to NP_003232.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-NQPLQSYHQLKLEFS, from the internal region (near N Terminus) of the protein sequence according to NP_003232.2.
Epitope
Internal region (near N Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,145 Da
NCBI Official Full Name
protein-glutamine gamma-glutamyltransferase 4
NCBI Official Synonym Full Names
transglutaminase 4
NCBI Official Symbol
TGM4
NCBI Official Synonym Symbols
TGP; hTGP
NCBI Protein Information
protein-glutamine gamma-glutamyltransferase 4
UniProt Protein Name
Protein-glutamine gamma-glutamyltransferase 4
Protein Family
UniProt Gene Name
TGM4
UniProt Synonym Gene Names
TG(P); TGP; TGase P; TGase-4
UniProt Entry Name
TGM4_HUMAN
Uniprot Description
TGM4: Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract. Belongs to the transglutaminase superfamily. Transglutaminase family.
Protein type: EC 2.3.2.13; Transferase
Chromosomal Location of Human Ortholog: 3p22-p21.33
Cellular Component: cytoplasm; extracellular matrix; Golgi apparatus
Molecular Function: metal ion binding; protein-glutamine gamma-glutamyltransferase activity
Biological Process: peptide cross-linking
Research Articles on TGM4
Similar Products
Product Notes
The TGM4 tgm4 (Catalog #AAA422770) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-transglutaminase 4 (aa49-63) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's transglutaminase 4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 77.1kDa according to NP_003232.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the TGM4 tgm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NQPLQSYHQL KLEFS. It is sometimes possible for the material contained within the vial of "transglutaminase 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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