Canine Allograft Inflammatory Factor 1 ELISA Kit | AIF1 elisa kit

Canine Allograft Inflammatory Factor 1 ELISA Kit

Cat. Num. AAA743957

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Canine
• Synonyms: Allograft Inflammatory Factor 1; Canine Allograft Inflammatory Factor 1 ELISA Kit; AIF1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This BG AIF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AIF1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This BG AIF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AIF1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIF1 elisa kit

Principle of the Assay: AIF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AIF1 antibody and an AIF1-HRP conjugate. The assay sample and buffer are incubated together with AIF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIF1 concentration since AIF1 from samples and AIF1-HRP conjugate compete for the anti-AIF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AIF1 from the sample, fewer sites are left to bind AIF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIF1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AIF1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 49457302
• NCBI GeneID: 199
• UniProt Accession #: Q9UIV4; O43904; Q9UKS9; A8K406; P55008
• Molecular Weight: 14,748 Da
• NCBI Official Full Name: AIF1, partial
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; perikaryon; actin filament; cytosol; nucleus; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; response to electrical stimulus; phagocytosis, engulfment; inflammatory response; negative regulation of apoptosis