Porcine Acyl-CoA desaturase (SCD) ELISA Kit | SCD elisa kit

Porcine Acyl-CoA desaturase (SCD) ELISA Kit

Cat. Num. AAA7210090

• Gene Names: SCD; SCD1; FADS5; SCDOS; MSTP008
• Reactivity: Porcine
• Synonyms: Acyl-CoA desaturase (SCD); Porcine Acyl-CoA desaturase (SCD) ELISA Kit; SCD elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of SCD. No significant cross-reactivity or interference between SCD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCD and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for SCD elisa kit

Intended Uses: This SCD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of PorcineSCD. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: SCD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCD antibody and an SCD-HRP conjugate. The assay sample and buffer are incubated together with SCD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCD concentration since SCD from samples and SCD-HRP conjugate compete for the anti-SCD antibody binding site. Since the number of sites is limited, as more sites are occupied by SCD from the sample, fewer sites are left to bind SCD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCD concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53759151
• NCBI GeneID: 6319
• NCBI Accession #: NP_005054.3
• NCBI GenBank Nucleotide #: NM_005063.4
• Molecular Weight: 41,523 Da
• NCBI Official Full Name: acyl-CoA desaturase
• NCBI Official Synonym Full Names: stearoyl-CoA desaturase (delta-9-desaturase)
• NCBI Official Symbol: SCD
• NCBI Official Synonym Symbols: SCD1; FADS5; SCDOS; MSTP008
• NCBI Protein Information: acyl-CoA desaturase; delta-9 desaturase; delta-9-desaturase; delta(9)-desaturase; fatty acid desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase opposite strand
• UniProt Protein Name: Acyl-CoA desaturase
• Protein Family: Acyl-CoA desaturase
• UniProt Gene Name: SCD
• UniProt Synonym Gene Names: Delta-9 desaturase
• UniProt Entry Name: ACOD_HUMAN

NCBI Description

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Feb 2012]

Uniprot Description

SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.

Protein type: EC 1.14.19.1; Endoplasmic reticulum; Oxidoreductase; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: iron ion binding; stearoyl-CoA 9-desaturase activity

Biological Process: fatty acid biosynthetic process