Human Acyl-CoA desaturase ELISA Kit | SCD elisa kit

Human Acyl-CoA desaturase ELISA Kit

Cat. Num. AAA2886780

• Gene Names: SCD; SCD1; FADS5; SCDOS; hSCD1; MSTP008
• Reactivity: Human
• Synonyms: Acyl-CoA desaturase; Human Acyl-CoA desaturase ELISA Kit; Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase; Stearoyl-CoA desaturase; SCD; 1.14.19.1; SCD elisa kit

• Reactivity: Human
• Sequence Length: 359

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCD elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53759151
• NCBI GeneID: 6319
• NCBI Accession #: NP_005054.3
• NCBI GenBank Nucleotide #: NM_005063.4
• UniProt Accession #: O00767; Q16150; Q53GR9; Q5W037; Q5W038; Q6GSS4; Q96KF6; Q9BS07; Q9Y695; B2R5U0; D3DR68
• Molecular Weight: 41,523 Da
• NCBI Official Full Name: acyl-CoA desaturase
• NCBI Official Synonym Full Names: stearoyl-CoA desaturase (delta-9-desaturase)
• NCBI Official Symbol: SCD
• NCBI Official Synonym Symbols: SCD1; FADS5; SCDOS; hSCD1; MSTP008
• NCBI Protein Information: acyl-CoA desaturase
• UniProt Protein Name: Acyl-CoA desaturase
• Protein Family: Acyl-CoA desaturase
• UniProt Gene Name: SCD
• UniProt Synonym Gene Names: hSCD1
• UniProt Entry Name: ACOD_HUMAN

NCBI Description

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]

Uniprot Description

SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.

Protein type: Oxidoreductase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral; EC 1.14.19.1

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: iron ion binding; oxidoreductase activity; stearoyl-CoA 9-desaturase activity

Biological Process: cellular lipid metabolic process; triacylglycerol biosynthetic process; unsaturated fatty acid biosynthetic process