Mouse Proopiomelanocortin ELISA Kit | POMC elisa kit

Mouse Proopiomelanocortin ELISA Kit

Cat. Num. AAA764937

• Gene Names: POMC; LPH; MSH; NPP; POC; ACTH; CLIP
• Reactivity: Mouse
• Synonyms: Proopiomelanocortin; Mouse Proopiomelanocortin ELISA Kit; POMC/Pro-opiomelanocortin/Corticotropin-lipotropin; POMC elisa kit

• Reactivity: Mouse
• Sequence Length: 30

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for POMC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse POMC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse POMC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse POMC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse POMC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 998476
• NCBI GeneID: 5443
• Molecular Weight: 29,424 Da
• NCBI Official Full Name: proopiomelanocortin, partial
• NCBI Official Synonym Full Names: proopiomelanocortin
• NCBI Official Symbol: POMC
• NCBI Official Synonym Symbols: LPH; MSH; NPP; POC; ACTH; CLIP
• NCBI Protein Information: pro-opiomelanocortin
• UniProt Protein Name: Pro-opiomelanocortin
• Protein Family: Pro-opiomelanocortin
• UniProt Gene Name: POMC
• UniProt Synonym Gene Names: POMC; ACTH; CLIP
• UniProt Entry Name: COLI_HUMAN

NCBI Description

This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jan 2016]

Uniprot Description

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; extracellular region; extracellular space; peroxisomal matrix; peroxisome; secretory granule

Molecular Function: G-protein-coupled receptor binding; hormone activity; receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor binding

Biological Process: cell-cell signaling; cellular pigmentation; cellular protein metabolic process; generation of precursor metabolites and energy; glucose homeostasis; negative regulation of tumor necrosis factor production; neuropeptide signaling pathway; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; regulation of appetite; regulation of blood pressure; signal transduction

Disease: Obesity; Proopiomelanocortin Deficiency